Phospho- ser/thr pka substrate antibody

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August undefined, 2024

WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP … WebImmunogen was a synthetic phospho-PKA substrate peptide. Phospho- (Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine …

Differential changes in cyclic adenosine 3′‐5′ monophosphate …

WebPhospho- (Ser/Thr) PKA Substrate Antibody can be used in high throughput kinase assays and drug discovery applications. It detects proteins and peptides containing a phospho … WebPhospho- (Ser/Thr) PKA Substrate Antibody. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. dan fishbach

Phosphorylation of Beet black scorch virus coat protein by PKA is ...

WebPhospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, … WebInactivation of a Gαs–PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis WebAnti-Phospho - (Ser/Thr) antibody Description Rabbit polyclonal to Phospho - (Ser/Thr) Host species Rabbit Tested applications Suitable for: ELISA, Dot blot more details Species … birmingham high school bell schedule

How to monitor PKA activity by western blot? ResearchGate

A single phosphorylation site of SIK3 regulates daily sleep ... - PNAS

WebDec 15, 2009 · (C) Thr-90-phosphorylation status of Hsp90α in the CM and TCL. pT90-Hsp90α was detected using anti-phospho-(Ser/Thr) PKA substrate antibody. (D) Secretion and Thr-90-phosphorylation status of Hsp90α upon overexpression of Flag-tagged PKA (Left) or upon siRNA treatment (Right) detected by Western blotting and ELISA. PKA was … WebThe NeuroMab™ anti-Phospho-PKA C (Thr197) antibody detects endogenous levels of PKA C (-α, -β, and -γ) only when phosphorylated at Thr197. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr197 of human PKA C protein. Store at -20°C. Do not aliquot the antibody. dan finney gold canyonWebIn order to study the phosphorylated proteins in the resting Xenopus laevis oocytes, the proteins detected by Western blotting using phospho-(Ser/Thr) PKA substrate antibody … dan fishback

"WebMay 20, 2024 · Epigallocatechin-3-gallate (EGCG) has widespread effects on adipocyte development. However, the molecular mechanisms of EGCG are not fully understood. We investigate the adipogenic differentiation of human-derived mesenchymal stem cells, including lipid deposition and changes in the expression and phosphorylation of key … " - Phospho- ser/thr pka substrate antibody

Phospho- ser/thr pka substrate antibody

PTMScan Phospho-PKA Substrate - Cell Signaling Technology

WebThis antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. Visit thermofisher.com to view references and figures. WebPhospho-(Ser/Thr) PKA Substrate Antibody 可检测包含磷酸-丝氨酸/苏氨酸残基（在 -3 位点有精氨酸）的肽和蛋白。它是一种有用的工具，可用来识别 AGC 家族激酶的底物，包括 …

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WebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations WebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also …

Web2 days ago · Site specific custom made phospho-Ser 932 PHLPP1 antibody was generated by GenScript (Genscript, USA) [12]. Membranes were washed in TBS-T and incubated with horseradish peroxidase-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad, 170-6515) secondary antibodies for 1 h at room temperature. WebA good control is the effect of the H89 inhibitor (as well as a known agonist such as dibutyryl cAMP). A possible PKA substrates is CREB (Ser 133) for which there are good phospho-specific antibodies.

WebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For … WebThe inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.

WebPhospho-(Ser/ Thr) PKA antibody substrates include Apaf-1, CAMKII, Caspase-10, Caspase-2, CREB, and NMDAR1, among others (see Cell Signaling #9621). Alpha--tubulin antibody was purchased from ...

WebPhospho-PKA substrate antibody AD0182 (supplied by Cell Signaling Technology) Phospho-PKA substrate antibody is an affinity-purified rabbit polyclonal antibody. It is highly specific for phosphorylated threonine with arginine at the –3 position. It also recognizes phospho-serine with arginine at the –2 and –3 position. birmingham high school californiaWebMar 27, 2024 · In healthy myocardium, PLB is phosphorylated at Ser 16 by PKA and at Thr 17 by CaMKII. 38, 39 Phosphorylation of TnI by PKA and PKC occurs at both Ser 23 and Ser 24 and leads to the depression of myofilament Ca 2+ sensitivity. 40-46 We tested whether these phosphorylation events were altered in the diabetic myocardium. dan fishback facebookWebNov 2, 2024 · Proteins retained on sepharose were incubated with an active recombinant PKA Cα subunit for the kinase assay and then blotted with an anti-phospho- (Ser/Thr) PKA substrate antibody or anti-myc... dan fischer usa todayWebMonoclonal Antibody for studying PKA Substrate motif [RXX(t/s)] phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA … dan firth sierra clubWebSep 9, 2005 · Western blot using a phospho-Thr antibody of GST-EEA1 (1257–1411) wt or T1392A (1 μg) after phosphorylation with MKK6DD-activated p38α or MKK6DD alone (right panels). ( B ) Endogenous EEA1 was immunoprecipitated from MKK6DD-expressing HEK293 cells (left panel) or SH-SY5Y cells (right panel) and blotted with antibodies against EEA1 … dan fischer one last waveWebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . dan fischer excavatingWeb3 a 1,2,3 875 879 9621 Phospho-(Ser/Thr) PKA Substrate Antibody 76 3 a 4,5,6 1170 1169 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody 140 3 a 7,8,9 1176 1175 9631 Phospho-(Ser/Thr) Phe Antibody 20 3 a 10,11,12 intentionally left blank 3 b … birmingham high school lake balboa