WebThe uptake of choline can occur via two transport systems, one that exhibits a high affinity for choline uptake (HAChU, K m =1–5 μM) and is dependent on both Na + and Cl −, and a second transporter system that has a significantly lower affinity for choline uptake (LAChU, K m =10–100 μM) and is independent of Na + and Cl − (see also Ch. 3). WebProtein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. …
Pseudomonas syringae BetT Is a Low-Affinity Choline
WebE. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA … Web23 de dez. de 2009 · We identified a choline, betaine and carnitine transporter, designated Cbc, from Pseudomonas syringae and Pseudomonas aeruginosa that is unusual among members of the ATP-binding cassette (ABC) transporter family in its use of multiple periplasmic substrate-binding proteins (SBPs) that are highly specific for their … list of teaching hospitals in texas
The high-affinity choline transporter: a critical protein for ...
Web29 de mar. de 2024 · This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from … Web1 de mar. de 2007 · For osmoprotection, Escherichia coli can synthesize glycine betaine from externally supplied choline by the Bet system (betTIBA products). The major carrier of choline is the high-affinity, proton-driven, secondary transporter BetT, which belongs to the BCCT family of transporters. Fusion proteins consisting of N-terminal fragments of … WebCholine uptake High affinity Na+-dependent Synaptosomes Certain basic characteristics of choline uptake in nerve terminals were ... protein, 0,567 uM [3H]-choline (final concentration), for 20 min at 371. The KM value of [3H]-choline uptake was 2.27+0.26 10 20 30 40 Time (min) 60 B I immigration fingerprint office near me