Differentiated sh-sy5y
WebSep 11, 2013 · (a) Lysates of SH-SY5Y cells, undifferentiated or differentiated with IGF-1 or RA were run on 12% SDS-PAGE gels and probed for differentiation marker proteins, β3 tubulin and GAP43, followed by detection with LI-COR Odyssey™. Densitometry of protein bands was measured using LI-COR Odyssey™ software and normalised to cellular … WebFeb 17, 2016 · The differentiation state of SH-SY5Y cells was undefined and ranged from tumor tissue state (neuroblastoma) to neural progenitor cells or post-mitotic neurons (Feles et al., 2024). Although ...
Differentiated sh-sy5y
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WebFeb 17, 2016 · In further support that differentiated SH-SY5Y cells represent a homogeneous neuronal population, removal of BDNF results in cellular apoptosis 4. This … WebSome differentiated SH-SY5Y cells are more suitable PD cell models than others, because they stop proliferating, have more DAergic neuronal properties, and have similar susceptibility to neurotoxins and neuroprotective agents as primary neurons. Although differentiated SH-SY5Y cells described to date fall slightly short of the ideal PD cell ...
WebDifferentiated SH-SY5Y DJ-1 WT ΔH8 and SH-SY5Y DJ-1 C106A ΔH8 cells were washed three times with phosphate buffered saline (PBS), harvested with a cell scraper, and lysed on ice in lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.02% SDS, 1 mM EDTA) with protease inhibitors (10 µM E64d, 1 mM … WebMar 19, 2024 · C2C12 cells were differentiated in the presence of the indicated amount of carnosine. Culture supernatants of carnosine-treated C2C12 cells were added to SH-SY5Y cells, and neurite outgrowth in SH-SY5Y cells was evaluated using IN Cell Analyzer 2200. As a positive control, SH-SY5Y cells were cultured with 10 μM of retinoic acid (RA) for 1 …
Web提供全反式维甲酸诱导分化对sh-sy5y细胞拮抗细胞毒性研究的影响文档免费下载，摘要:实验研究论著全反式维甲酸诱导分化对shsy5y细胞拮抗细胞毒性研究的影响张玉柯王振华陈姬张娟郑秋生新疆特种植物药资源教育部重点实验室石河子大学药学院新疆石河子832002摘要:[目的]观察全反式维甲酸(ra)诱导 ... WebApr 13, 2024 · Differentiated SH-SY5Y cells have been recently used for imaging the movement of synaptic vesicles by single-molecule localization microscopy . Our protocol shows the suitability of this system for studying long-range cotransport of mitochondria and nucleoids by live super-resolution imaging. The method also lends itself to studying the ...
WebJul 31, 2024 · Differentiated SH-SY5Y cells were collected from 24-well culture plates at 2–3 weeks in culture. Before cells were transferred to the recording chamber under an upright Nikon Eclipse FN1 microscope, culture medium was gradually changed to oxygenated artificial cerebral spinal fluid (ACSF) containing ...
WebMoreover, MC was protective against 6-OHDA-evoked neurotoxicity in neuronal differentiated SH-SY5Y cells via inhibition of necrotic and apoptotic processes. On the … lcm of 7 and 97WebJan 1, 2013 · 1.2 Undifferentiated Versus Differentiated SH-SY5Y Cells. The ability for researchers to differentiate SH-SY5Y neuroblastoma cells into cells possessing a more mature, neuron-like phenotype through manipulation of the culture medium has afforded numerous benefits in the field of neuroscience research. Advantages include the … lcm of 7 and 2WebApr 12, 2024 · Neurofilament dynamics were studied in primary DRG neurons and differentiated SH-SY5Y cells. On day seven of primary DRG neuron culture NFM dynamics was recorded for 30 min with interval of 0.5 or 1 min after 5 s photoconversion. Undifferentiated SH-SY5Y cells were electroporated with mEOS_NFM in pQCXIN … lcm of 7 and 11 and 21 and 22