Cst atf4 antibody

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August undefined, 2024

WebPurified anti-ATF4 Antibody. Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, six hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor's antibody used at the ... WebDec 19, 2024 · Shipping Temperature : Blue Ice or 4ºC. Cite this Product : StressMarq Biosciences Cat# SMC-447D, RRID: AB_2701793. Certificate of Analysis : 1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Oct-4 Antibody Cell Signaling Technology

WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. WebOverview Product name Anti-ATF-4 antibody See all ATF-4 primary antibodies Description Rabbit polyclonal to ATF-4 Host species Rabbit … china states names

Anti-ATF-4 (phospho S245) antibody (ab28830) Abcam

WebSep 29, 2024 · ATF-4 Antibody (B-3) is an IgG 2b κ mouse monoclonal ATF-4 antibody (also designated ATF4 antibody, cAMP-dependent transcription factor ATF-4 antibody, CREB-2 antibody or cAMP … WebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … WebATF-4 Antibodies Santa Cruz Biotechnology, Inc. offers a broad range of ATF-4 antibodies. Select ATF-4 antibodies from monoclonal antibodies listed below. View detailed ATF-4 … china states map

Anti-ATF4 Antibody Products from Cell Signaling Technology

WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo … WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these … grammy play off musicWebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously … grammy pop nominations

"WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and … " - Cst atf4 antibody

Cst atf4 antibody

ATF4 Antibody StressMarq Biosciences SMC-447D product …

WebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ... WebJun 14, 2013 · The transcription factor activating transcription factor 4 (ATF4) belongs to the cAMP-responsive element–binding protein family, characterized by the presence of a leucine zipper dimerization domain and a basic amino acid–rich DNA binding domain ().Previous studies have shown that knocking out ATF4 gene expression in mice results …

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WebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype … WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit …

WebResearch studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the … WebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR …

WebActivating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor … WebJan 10, 2024 · Moreover, ATF4 overexpression inhibited tumor proliferation and AR expression both in vitro and in vivo. Collectively, these results demonstrated that ER …

WebFeb 17, 2024 · Autophagy is a cellular process through which cells engulf and degrade damaged cytoplasmic components ().Recent studies have implicated the involvement of autophagy in the regulation of obesity and energy expenditure (15–21).For example, autophagy is reduced in the hypothalamus of aged mice or mice fed an HFD …

WebMar 3, 2024 · Effective treatments for patients suffering from heat hypersensitivity are lacking, mostly due to our limited understanding of the pathogenic mechanisms underlying this disorder. In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and … grammy predictions 2022Webab31390 has been referenced in 9 publications. Liu Y Hydrogen peroxide induces nucleus pulposus cell apoptosis by ATF4/CHOP signaling pathway. Exp Ther Med 20:3244-3252 (2024). PubMed: 32855694; Liu P et al. … grammy predictions billboardWebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti … grammy predictionsWeb1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (red), or 0.4 ug Control … china stationery ltdWebActivating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding … grammy pre telecastWebThis antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated … grammy predictions 2023WebMar 25, 2024 · Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (56583). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (56583) at … grammy playlist